Journal: Arquivos Brasileiros de Cardiologia

Article Title: Effects of Chronic Exercise on Endothelial Progenitor Cells and Microparticles in Professional Runners

doi: 10.5935/abc.20170022

Figure Lengend Snippet: Box-plots showing the percentage of circulating endothelial progenitor cells (EPCs) determined by flow-cytometry. Higher percentage of CD34+/KDR+ EPCs (A) (p=0.038 vs. controls, Mann-Whitney U test), as well as CD133+/KDR+ EPCs (p=0.018 vs. controls, Mann-Whitney U test) (B) were found in athletes. No differences were observed between groups for CD133+/CD34+ (p=0.51) (C).

Article Snippet: Fluorescently labeled mouse anti-human antibodies were used for EPCs (CD34 FITC, BD Biosciences, USA; CD133 APC, Miltenyi Biotec, USA; KDR PE, R&D Systems, USA), PMPs (CD42 FITC and CD31 PE, BD Biosciences, USA) and EMPs (CD51 FITC, BD Biosciences).

Techniques: Flow Cytometry, MANN-WHITNEY

Journal: Molecular Medicine Reports

Article Title: Irradiation alters the differentiation potential of bone marrow mesenchymal stem cells

doi: 10.3892/mmr.2015.4539

Figure Lengend Snippet: CFUs of BMSCs following irradiation. Giemsa staining of the BMSCs following irradiation at (A) 0 Gy, (B) 0.5 Gy, (C) 1 Gy, (D) 2 Gy, (E) 5 Gy, (F) 10 Gy. Original magnification, ×20. The graph shows the number of CFUs following irradiation with doses of 0, 0.5, 1, 2, 5 and 10 Gy. Data are expressed as the mean ± standard deviation * P<0.05, compared with the 0 Gy group. BMSC, bone marrow mesenchymal stem cell; CFU, colony-forming unit.

Article Snippet: Rat polyclonal anti-CD34-fluorescein isothiocyanate (1:1,000; cat. no. bs-2038R; FITC; Bioss Biosynthesis Biotechnology Co., Ltd., Beijing, China), rat monoclonal anti-CD29-FITC (1:1,000; cat. no. 555005; BD Biosciences, San Jose, CA, USA), rat polyclonal anti-CD-44-FITC (1:1,000; cat. no. FAB6577G; R&D Systems, Inc., Minneapolis, MN, USA) and rat anti-CD45-FITC (1:500; cat. no. 554877; BD Biosciences) antibodies were added separately, followed by 30 min incubation in the dark at 4°C.

Techniques: Irradiation, Staining, Standard Deviation

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors

doi: 10.1016/j.ajps.2022.07.002

Figure Lengend Snippet: In vivo immune responses assessment. (A) Flow cytometry assay of CD80 + or CD86 + gated on CD11c + in the TDLNs of mice. (B) Flow cytometry assay illustrated the percent of CD8a + T cells and CD4 + T cells gated on CD3 + T cells in splenocytes of mice. (C-D) Quantitative percentages of CD80 + or CD86 + expressions gated on CD11c + according to (A). (E) Quantitative percentages of CD8a + T cells and CD4 + T cells in splenocytes according to (B). (F-H) Quantitative analysis of the immune-associated cytokines in serum, as determined by ELISA (IL-2, TNF-α and IFN-γ). ( n = 3, mean ± SD, * P < 0.05, ** P < 0.01 and ## P < 0.0001).

Article Snippet: Fluorochrome-labeled anti-mouse monoclonal antibodies (CD11c-allophycocyanin (APC), CD80-fluorescein isothiocyanate (FITC), CD86-phycoerythrin (PE), CD3-FITC, CD8a-PE, and CD4-APC) were bought from Proteintech.

Techniques: In Vivo, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model

doi: 10.3390/ijms23063310

Figure Lengend Snippet: Flow cytometry analysis of isolated porcine Wharton´s jelly mesenchymal stem cells (pWJCs).

Article Snippet: 34 , 0.38% , Biorbyt (Cambridge, UK) , orb247244.

Techniques: Flow Cytometry, Isolation, Marker